A bacterial haloalkane dehalogenase gene as a negative selectable marker in Arabidopsis
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A bacterial haloalkane dehalogenase gene as a negative selectable marker in Arabidopsis.
The dhlA gene of Xanthobacter autotrophicus GJ10 encodes a dehalogenase which hydrolyzes dihalo- alkanes, such as 1, 2-dichloroethane (DCE), to a halogenated alcohol and an inorganic halide (Janssen et al. 1994, Annu. Rev. Microbiol. 48, 163-191). In Xanthobacter, these alcohols are further catabolized by alcohol and aldehyde dehydrogenase activities, and by the product of the dhlB gene to a se...
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DmmA is a haloalkane dehalogenase (HLD) identified and characterized from the metagenomic DNA of a marine microbial consortium. Dehalogenase activity was detected with 1,3-dibromopropane as substrate, with steady-state kinetic parameters typical of HLDs (K(m) = 0.24 ± 0.05 mM, k(cat) = 2.4 ± 0.1 s(-1) ). The 2.2-Å crystal structure of DmmA revealed a fold and active site similar to other HLDs...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1999
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.1999.00477.x